SPHK1 Protein (His-GST)
Quick Overview for SPHK1 Protein (His-GST) (ABIN7317044)
Target
See all SPHK1 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This SPHK1 protein is labelled with His-GST.
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Purpose
- Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (His&GST Tag)
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Sequence
- Met 1-Leu 384
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Characteristics
- A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Sphingosine Kinase 1 (SPHK1) (AA 1-384) protein (His tag)
custom-made SPHK1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Sphingosine Kinase 1 (SPHK1) (AA 1-382) protein (His tag)custom-made SPHK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Sphingosine Kinase 1 (SPHK1) (AA 1-384) protein (GST tag)SPHK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sphingosine Kinase 1 (SPHK1) (Active) proteinSPHK1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10 % glycerol, pH 8.0
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- SPHK1 (Sphingosine Kinase 1 (SPHK1))
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Alternative Name
- SPHK1/Sphingosine Kinase 1
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Background
- SPHK,SPHK1,SPK,SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.
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Molecular Weight
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Calculated MW: 70.3 kDa
Observed MW: 64 kDa
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Gene ID
- 8877
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UniProt
- Q9NYA1
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Pathways
- VEGF Signaling
Target
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