SPHK1 Protein
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- Target See all SPHK1 Proteins
- SPHK1 (Sphingosine Kinase 1 (SPHK1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purpose
- Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (Active)
- Sequence
- Met 1-Leu 384
- Characteristics
- A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus.
- Purity
- > 85 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.
- Top Product
- Discover our top product SPHK1 Protein
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Sphingosine Kinase 1 (SPHK1) (AA 1-384) (Active) protein (GST tag)
SPHK1 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
Sphingosine Kinase 1 (SPHK1) (AA 1-384) protein (GST tag)SPHK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sphingosine Kinase 1 (SPHK1) (AA 261-353) protein (GST tag)SPHK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sphingosine Kinase 1 (SPHK1) protein (GST tag,His tag)SPHK1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Sphingosine Kinase 1 (SPHK1) (AA 1-384) protein (Strep Tag)Crystallography grade SPHK1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Sphingosine Kinase 1 (SPHK1) (AA 1-382) protein (Strep Tag)Crystallography grade SPHK1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Sphingosine Kinase 1 (SPHK1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SPHK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- SPHK1 (Sphingosine Kinase 1 (SPHK1))
- Alternative Name
- SPHK1/Sphingosine Kinase 1 (SPHK1 Products)
- Synonyms
- CG1747 Protein, Dmel\\CG1747 Protein, SK1 Protein, Sphk1 Protein, SPHK1 Protein, F18E5.160 Protein, F18E5_160 Protein, sphingosine kinase 1 Protein, SPHK Protein, 1110006G24Rik Protein, Sk1 Protein, Spk1 Protein, Sphingosine kinase 1 Protein, sphingosine kinase 1 Protein, sphingosine kinase 1 L homeolog Protein, Sk1 Protein, SPHK1 Protein, sphk1.L Protein, Sphk1 Protein
- Background
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Background: SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.
Synonym: SPHK,SPHK1,SPK
- Molecular Weight
- 42.7 kDa
- Pathways
- VEGF Signaling
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