SOCS3 Protein (His-TRX)

Catalog No. ABIN7317579

This Recombinant SOCS3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SOCS3 Protein (His-TRX) (ABIN7317579)

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This SOCS3 protein is labelled with His-TRX.

Purpose: Recombinant Human SOCS3/CIS3 Protein (His & Trx Tag)

Sequence: Met 1-Leu 225

Characteristics: A DNA sequence encoding the human SOCS3 (O14543) (Met 1-Leu 225) was fused with a Trx and a polyhistide tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Alternative Name: SOCS3/CIS3

Background: ATOD4,CIS3,Cish3,SOCS-3,SSI-3,SSI3,Suppressor of cytokine signaling 3, also known as SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3, SOCS3 and CIS3, is a protein which is widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. SOCS3 / CIS3 contains oneSH2 domain and oneSOCS box domain. SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 / CIS3 is involved in negative regulation of cytokines that signal through the JAK / STAT pathway. SOCS3 / CIS3 inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. SOCS3 / CIS3 suppresses fetal liver erythropoiesis. It regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. SOCS3 / CIS3 regulates IL-6 signaling. SOCS3 / CIS3 interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. SOCS3 / CIS3 could be used as a possible therapeutic agent for treating rheumatoid arthritis.

Molecular Weight:

Calculated MW: 41.9 kDa

Observed MW: 46 kDa

Gene ID: 9021

UniProt: O14543

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus, Hepatitis C