TEK Protein (His tag)
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- Target See all TEK Proteins
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TEK protein is labelled with His tag.
- Purpose
- Recombinant Mouse Tie2/CD202b Protein (His Tag)(Active)
- Sequence
- Met1-Lys744
- Characteristics
- A DNA sequence encoding the mouse TEK (Q02858) (Met1-Lys744) was expressed with a C-terminal polyhistidine tag.
- Purity
- > 95 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized mouse TEK-His at 10 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 6.25-200 ng/ml.
- Top Product
- Discover our top product TEK Protein
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TEK Tyrosine Kinase, Endothelial (TEK) (AA 23-748) (Active) protein (Fc Tag)
TEK Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
TEK Tyrosine Kinase, Endothelial (TEK) (Active) protein (Fc Tag)TEK Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
TEK Tyrosine Kinase, Endothelial (TEK) (AA 770-1122) (Active) protein (GST tag,His tag)TEK Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 91 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
- Alternative Name
- Tie2/CD202b (TEK Products)
- Background
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Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Synonym: AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2
- Molecular Weight
- 82.4 kDa
- UniProt
- Q02858
- Pathways
- RTK Signaling, Growth Factor Binding
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