TEK Protein

Catalog No. ABIN7520536

Product Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purpose: Active Recombinant Human TIE2/TEK/CD202b Protein

Sequence: KNNPDPTIYP VLDWNDIKFQ DVIGEGNFGQ VLKARIKKDG LRMDAAIKRM KEYASKDDHR DFAGELEVLC KLGHHPNIIN LLGACEHRGY LYLAIEYAPH GNLLDFLRKS RVLETDPAFA IANSTASTLS SQQLLHFAAD VARGMDYLSQ KQFIHRDLAA RNILVGENYV AKIADFGLSR GQEVYVKKTM GRLPVRWMAI ESLNYSVYTT NSDVWSYGVL LWEIVSLGGT PYCGMTCAEL YEKLPQGYRL EKPLNCDDEV YDLMRQCWRE KPYERPSFAQ ILVSLNRMLE ERKTYVNTTL YEKFTYAGID CSAEEAA

Specificity: Lys808-Ala1124

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human TEK Protein at 2 μg/mL (100 μL/well) can bind ANG2 with a linear range of 39-7090 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE2/TEK/CD202b (TEK Products)

Synonyms: TEK Protein, DKFZp469E064 Protein, AA517024 Protein, Cd202b Protein, Hyk Protein, Tie2 Protein, tie-2 Protein, CD202B Protein, TIE-2 Protein, TIE2 Protein, VMCM Protein, VMCM1 Protein, Tie-2 Protein, tie2 Protein, TEK receptor tyrosine kinase Protein, angiopoietin-1 receptor Protein, TEK tyrosine kinase, endothelial Protein, TEK Protein, LOC100408334 Protein, tek Protein, Tek Protein

Background:

Description: Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts.

Name: TEK,CD202B,GLC3E,TIE-2,TIE2,VMCM,VMCM1,Tie2

Gene ID: 7010

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding