TEK Protein (Fc Tag)

Catalog No. ABIN7198206

Product Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This TEK protein is labelled with Fc Tag.

Purpose: Recombinant Rat Tie2/TEK Protein (Fc Tag)(Active)

Sequence: Met 4-Leu 743

Characteristics: A DNA sequence encoding the rat TEK (NP_001099207.1) extracellular domain (Met 4-Leu 743) was fused with the Fc region of human IgG1 at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized S1h-3C-mANGPT2 at 10 μg/mL (100 μL/well) can bind ratTEK-Fc.The EC50 of ratTEK-Fc is 0.26-0.62μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: Tie2/TEK (TEK Products)

Synonyms: TEK Protein, DKFZp469E064 Protein, AA517024 Protein, Cd202b Protein, Hyk Protein, Tie2 Protein, tie-2 Protein, CD202B Protein, TIE-2 Protein, TIE2 Protein, VMCM Protein, VMCM1 Protein, Tie-2 Protein, tie2 Protein, TEK receptor tyrosine kinase Protein, angiopoietin-1 receptor Protein, TEK tyrosine kinase, endothelial Protein, TEK Protein, LOC100408334 Protein, tek Protein, Tek Protein

Background:

Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Synonym: Tie-2,Tie2

Molecular Weight: 108 kDa

NCBI Accession: NP_001099207

Pathways: RTK Signaling, Growth Factor Binding