TEK Protein (AA 770-1122) (GST tag,His tag)

Catalog No. ABIN7320054

Product Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 770-1122

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This TEK protein is labelled with GST tag,His tag.

Purpose: Recombinant Mouse Tie2/CD202b Protein (aa 770-1122, His & GST Tag)(Active)

Sequence: Gln770-Ala1122

Characteristics: A DNA sequence encoding the mouse TEK (Q02858) (Gln770-Ala1122) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 91 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. No Kinase Activity2. Measured by its binding ability in a functional ELISA. Immobilized mouse TEK (aa 770-1122) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.25-2.0 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: Tie2/CD202b (TEK Products)

Synonyms: TEK Protein, DKFZp469E064 Protein, AA517024 Protein, Cd202b Protein, Hyk Protein, Tie2 Protein, tie-2 Protein, CD202B Protein, TIE-2 Protein, TIE2 Protein, VMCM Protein, VMCM1 Protein, Tie-2 Protein, tie2 Protein, TEK receptor tyrosine kinase Protein, angiopoietin-1 receptor Protein, TEK tyrosine kinase, endothelial Protein, TEK Protein, LOC100408334 Protein, tek Protein, Tek Protein

Background:

Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Synonym: AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2

Molecular Weight: 68.2kDa

UniProt: Q02858

Pathways: RTK Signaling, Growth Factor Binding