TXN Protein

Catalog No. ABIN7198219

Product Details

Target: TXN (Thioredoxin (TXN))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Thioredoxin/TXN Protein (Active)

Sequence: Met 1-Val 105

Characteristics: A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified.

Purity: > 97 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to catalyze the reduction of insulin. The specific activity is 5-9 pmoles/min/μg.2. Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TXN (Thioredoxin (TXN))

Alternative Name: Thioredoxin/TXN (TXN Products)

Synonyms: TRDX Protein, TRX Protein, TRX1 Protein, ADF Protein, AW550880 Protein, Trx1 Protein, Txn Protein, trx Protein, TXN Protein, SCM1.18 Protein, trxA3 Protein, thioredoxin Protein, TR-1 Protein, Trx-1 Protein, thioredoxin Protein, thioredoxin 1 Protein, thioredoxin L homeolog Protein, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Protein, thioredoxin pseudogene Protein, cytosolic thioredoxin Trx1 Protein, TXN Protein, Txn1 Protein, txn.L Protein, txn Protein, trx Protein, SCO0885 Protein, PHG25ORF099c Protein, LOC100349046 Protein, Txn Protein, trx1 Protein

Background:

Background: Thioredoxin; also known as ATL-derived factor; Surface-associated sulphydryl protein; SASP and TXN; is a nucleus; cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO); and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Synonym: Thioredoxin; Trx; ATL-Derived Factor; ADF; Surface-Associated Sulphydryl Protein; SASP; TXN; TRDX; TRX; TRX1

Molecular Weight: 11.7 kDa

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis