TLK2 Protein (His-GST)

Catalog No. ABIN7317072

This Recombinant TLK2 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for TLK2 Protein (His-GST) (ABIN7317072)

Target: TLK2 (Tousled-Like Kinase 2 (TLK2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This TLK2 protein is labelled with His-GST.

Purpose: Recombinant Human TLK2/PKU-ALPHA Protein (His&GST Tag)

Sequence: Leu 397-Asn 772

Characteristics: A DNA sequence encoding the amino acids (Leu 397-Asn 772) of human TLK2 (Q86UE8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 8.0

Preservative: PMSF

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: TLK2 (Tousled-Like Kinase 2 (TLK2))

Alternative Name: TLK2/PKU-ALPHA

Background: HsHPK,PKU-ALPHA,Serine / threonine-protein kinase tousled-like 2, also known as PKU-alpha, Tousled-like kinase 2 and TLK2, is a nucleus protein which belongs to theprotein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair, DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains oneprotein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

Molecular Weight:

Calculated MW: 71.3 kDa

Observed MW: 65 kDa

Gene ID: 11011

UniProt: Q86UE8