WARS Protein (His tag)
Quick Overview for WARS Protein (His tag) (ABIN7319122)
Target
See all WARS ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This WARS protein is labelled with His tag.
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Purpose
- Recombinant Human WARS/TrpRS Protein (His Tag)
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Sequence
- Met 1-Gln471
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Characteristics
- Recombinant Human Tryptophan--tRNA ligase, cytoplasmic is produced by our E.coli expression system and the target gene encoding Met1-Gln471 is expressed with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Tryptophanyl-tRNA Synthetase (WARS) (AA 1-471) protein (GST tag)
WARS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Tryptophanyl-tRNA Synthetase (WARS) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)WARS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Tryptophanyl-tRNA Synthetase (WARS) proteinWARS Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Tryptophanyl-tRNA Synthetase (WARS) proteinWARS Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 8 % Trehalose, 4 % Mannitol, 50 mM NaCl, 0.05 % Tween 80, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- WARS (Tryptophanyl-tRNA Synthetase (WARS))
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Alternative Name
- WARS/TrpRS
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Background
- Interferon-induced protein 53.,WARS also known as Tryptophanyl-tRNA synthetase,There exists two types of tryptophanyl tRNA synthetases, the cytoplasmic form called WARS, the mitochondrial form called WARS2. WARS catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. WARS regulates ERK, Akt, eNOS activation pathway, which are related with angiogenesis, cytoskelatal reorganization and shear stess-reponsive gene expression.
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Molecular Weight
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Calculated MW: 55.3 kDa
Observed MW: 60 kDa
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Gene ID
- 7453
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UniProt
- P23381
Target
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