USP7 Protein (AA 208-560) (His-GST)

Catalog No. ABIN7198567

This Recombinant USP7 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for USP7 Protein (AA 208-560) (His-GST) (ABIN7198567)

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 98 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 208-560

Purification tag / Conjugate: This USP7 protein is labelled with His-GST.

Purpose: Recombinant Human USP7/HAUSP Protein (aa 208-560, His & GST Tag)

Sequence: Lys 208-Glu 560

Characteristics: A DNA sequence encoding the amino acid sequence (Lys 208-Glu 560) of human USP7 (NP_003461.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Alternative Name: USP7/HAUSP

Background: HAUSP,TEF1,Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein which belongs to thepeptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP0 stimulates lytic infection and the reactivation of quiescent viral genomes. ICP0 interacts very strongly with USP7. USP7-mediated stabilization of ICP0 is dominant over ICP0-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP0-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP0 are expressed.

Molecular Weight:

Calculated MW: 68.8 kDa

Observed MW: 60 kDa

Gene ID: 7874

NCBI Accession: NP_003461

UniProt: Q93009