UBA1 Protein (His-GST)

Catalog No. ABIN7317442

Recombinant UBA1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for UBA1 Protein (His-GST) (ABIN7317442)

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 96 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This UBA1 protein is labelled with His-GST.

Purpose: Recombinant Human UBE1/UBA1 Protein (His & GST Tag)

Sequence: Ser 2-Arg 1058

Characteristics: A DNA sequence encoding the human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: UBE1/UBA1

Background: A1S9,A1S9T,A1ST,AMCX1,CFAP124,CTD-2522E6.1,GXP1,POC20,SMAX2,UBA1A,UBE1,UBE1X,UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

Molecular Weight:

Calculated MW: 146 kDa

Observed MW: 130 kDa

Gene ID: 7317

NCBI Accession: NP_003325

UniProt: P22314