Sonic Hedgehog Protein (SHH) (His tag)

Catalog No. ABIN7320899

This Recombinant Sonic Hedgehog protein is expressed in Human Cells.

Quick Overview for Sonic Hedgehog Protein (SHH) (His tag) (ABIN7320899)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purity: >95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Sonic Hedgehog protein is labelled with His tag.

Purpose: Recombinant Mouse Sonic Hedgehog/SHH (C-6His)

Sequence: Cys25--Gly198

Characteristics: Recombinant Mouse Sonic Hedgehog is produced by our Mammalian expression system and the target gene encoding Cys25-Gly198 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Comment:

22 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, 5 % Trehalose, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: SHH

Background:

Background: Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. Mouse Shh-N is 99 % , 98 %, and 100 % aa identical to human, rat and gerbil Shh-N, respectively.

Synonym: Sonic Hedgehog Protein, SHH, HHG-1, SHH

Molecular Weight: 20.4 kDa

UniProt: Q62226

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development