DDR1 Protein (GST tag,His tag)

Catalog No. ABIN7317090

This Recombinant DDR1 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for DDR1 Protein (GST tag,His tag) (ABIN7317090)

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 89 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This DDR1 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human DDR1 Kinase/MCK10 Protein (aa 444-913, His & GST Tag)(Active)

Sequence: Arg444-Val913

Characteristics: A DNA sequence encoding the human DDR1 (Q08345-1) (Arg444-Val913) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 2.75 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.

Application Details

Comment:

80 kDa

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 3 mM DTT

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Alternative Name: MCK10

Background:

Background: Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Synonym: CAK,CD167,DDR,EDDR1,HGK2,MCK10,NEP,NTRK4,PTK3,PTK3A,RTK6,TRKE

Molecular Weight: 80 kDa

Pathways: RTK Signaling, Smooth Muscle Cell Migration