DDR1 Protein (AA 21-417) (His tag)

Catalog No. ABIN7274460

This Recombinant DDR1 protein is produced in HEK-293 Cells.

Quick Overview for DDR1 Protein (AA 21-417) (His tag) (ABIN7274460)

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-417

Purification tag / Conjugate: This DDR1 protein is labelled with His tag.

Purpose: Human DDR1 Protein

Sequence: Asp21-Ala417

Characteristics: Recombinant Human DDR1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp21-Ala417.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200).,Immobilized Human DDR1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).,Human DDR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human DDR1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Alternative Name: DDR1

Background: Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways, however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.

Molecular Weight: 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Bis-Tris PAGE result.

UniProt: Q08345

Pathways: RTK Signaling, Smooth Muscle Cell Migration