CXCL12 Protein (Biotin)

Catalog No. ABIN7328940

This Recombinant CXCL12 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CXCL12 Protein (Biotin) (ABIN7328940)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA), Cell Migration Assay (CMA)

Purity: > 97 %

Product Details

Purification tag / Conjugate: This CXCL12 protein is labelled with Biotin.

Purpose: Recombinant human CXCL12a is produced in E. coli

Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNN RQVCIDPKLK WIQEYLEKAL NKKLGSGLND IFEAQKIEWH E

Characteristics: Recombinant human CXCL12a is produced in E. coli

Biological Activity Comment: EC50 = 2.5nm determined by Calcium Flux with recombinant human CXCR4 cells.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tube prior to resuspending. Recommended at 100 μg/mL in sterile distilled water.

Storage: -20 °C,-80 °C

Target Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12a

Background: Stromal cell-derived factor 1,CXCL12a and CXCL12b, also known as Stromal Cell-Derived Factor 1a and 1b (SDF-1a and SDF-1b), are small cytokines that belong to the intercrine family. Both forms of CXCL12 are produced by alternate splicing of the same gene. CXCL12 is strongly chemotactic for T- lymphocytes and monocytes, and it plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism. It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumor progression. CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12. In breast cancer, however, increased expression of CXCL12 is associated with a reduced risk of distant metastasis. By blocking CXCR4, a major coreceptor for HIV-1 entry, CXCL12 acts as an endogenous inhibitor of CXCR4-tropic HIV-1 strains. CXCL12 was shown to be expressed in many tissues in mice including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow.,rHuCXCL12a-biotin, SDF-1alpha

Molecular Weight: 10.4 kDa

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling