AMIGO2 Protein (AA 39-397) (His tag)

Catalog No. ABIN7273830

This Recombinant AMIGO2 protein is produced in HEK-293 Cells.

Quick Overview for AMIGO2 Protein (AA 39-397) (His tag) (ABIN7273830)

Target: AMIGO2 (Adhesion Molecule with Ig-Like Domain 2 (AMIGO2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 39-397

Purification tag / Conjugate: This AMIGO2 protein is labelled with His tag.

Purpose: Mouse AMIGO2 Protein

Sequence: Met39-Thr397

Characteristics: Recombinant Mouse AMIGO2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met39-Thr397.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse AMIGO2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse AMIGO2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: AMIGO2 (Adhesion Molecule with Ig-Like Domain 2 (AMIGO2))

Alternative Name: AMIGO2

Background: Bromodomain and extraterminal domain inhibitors (BETi) represent promising therapeutic agents for metastatic melanoma, yet their mechanism of action remains unclear. Here we interrogated the transcriptional effects of BETi and identified AMIGO2, a transmembrane molecule, as a BET target gene essential for melanoma cell survival. AMIGO2 is upregulated in melanoma cells and tissues compared to human melanocytes and nevi, and AMIGO2 silencing in melanoma cells induces G1/S arrest followed by apoptosis.

Molecular Weight: 41.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: Q80ZD9