Angiopoietin 2 Protein (ANGPT2) (AA 275-496) (Biotin,His-Avi Tag)

Catalog No. ABIN7273861

This Recombinant Angiopoietin 2 protein is expressed in HEK-293 Cells.

Quick Overview for Angiopoietin 2 Protein (ANGPT2) (AA 275-496) (Biotin,His-Avi Tag) (ABIN7273861)

Target: Angiopoietin 2 (ANGPT2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 275-496

Purification tag / Conjugate: This Angiopoietin 2 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human ANGPT2/Angiopoietin-2 Protein

Sequence: Lys275-Phe496

Characteristics: Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Lys275-Phe496.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 uM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human ANGPT2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).,Biotinylated Human ANGPT2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: ANGPT2

Background: Angiopoietin-2 (Ang-2, also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.

Molecular Weight: 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.

UniProt: O15123

Pathways: RTK Signaling