Aspartate beta Hydroxylase Protein (AA 324-741) (His tag)

Catalog No. ABIN7273879

This Recombinant Aspartate beta Hydroxylase protein is expressed in Escherichia coli (E. coli).

Quick Overview for Aspartate beta Hydroxylase Protein (AA 324-741) (His tag) (ABIN7273879)

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 324-741

Purification tag / Conjugate: This Aspartate beta Hydroxylase protein is labelled with His tag.

Purpose: Mouse ASPH Protein

Sequence: Ile324-Ile741

Characteristics: Recombinant Mouse ASPH Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ile324-Ile741.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse ASPH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse ASPH is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Alternative Name: ASPH

Background: Aspartate β-hydroxylase (ASPH) is silent in normal adult tissues only to re-emerge during oncogenesis where its function is required for generation and maintenance of malignant phenotypes. Exosomes enable prooncogenic secretome delivering and trafficking for long-distance cell-to-cell communication.Expression profiling of Notch signaling components positively correlates with ASPH expression in breast cancer patients, confirming that ASPH-Notch axis acts functionally in breast tumorigenesis.

Molecular Weight: 49.4 kDa same as Bis-Tris PAGE result.

UniProt: Q8BSY0

Pathways: Positive Regulation of Endopeptidase Activity