BCMA Protein (AA 1-53) (hFc Tag)

Catalog No. ABIN7273936

This Recombinant BCMA protein is expressed in HEK-293 Cells.

Quick Overview for BCMA Protein (AA 1-53) (hFc Tag) (ABIN7273936)

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-53

Purification tag / Conjugate: This BCMA protein is labelled with hFc Tag.

Purpose: Cynomolgus/Rhesus macaque BCMA/TNFRSF17 Protein

Sequence: Met1-Ala53

Characteristics: Recombinant Cynomolgus/Rhesus macaque BCMA/TNFRSF17 Protein is expressed from HEK293 with hFc (IgG1) at the C-Terminus.It contains Met1-Ala53.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).,Immobilized Cynomolgus/Rhesus macaque BCMA, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BCMA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.,Cynomolgus/Rhesus macaque BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Cynomolgus APRIL (Trimer), His-Avi Tag on Anti-His-Biosensor can bind Cynomolgus BCMA, hFc Tag with an affinity constant of 1.59 nM as determined in BLI assay (Gator® Prime).,The purity of Cynomolgus/Rhesus macaque BCMA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Alternative Name: BCMA

Background: B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

Molecular Weight: 31.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5UD97