BCMA Protein (AA 1-54) (Biotin,His-Avi Tag)

Catalog No. ABIN7273938

This Recombinant BCMA protein is produced in HEK-293 Cells.

Quick Overview for BCMA Protein (AA 1-54) (Biotin,His-Avi Tag) (ABIN7273938)

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 1-54

Purification tag / Conjugate: This BCMA protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human BCMA/TNFRSF17 Protein

Sequence: Met1-Ala54

Characteristics: Recombinant Biotinylated Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Ala54.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human BCMA, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).,Immobilized Human APRIL (Trimer), His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 38.2ng/ml determined by ELISA.,Immobilized Anti-BCMA Antibody, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA.,Biotinylated Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-BCMA antibody on ProA-Biosensor can bind Biotinylated Human BCMA, His-Avi Tag with an affinity constant of 4.09 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Alternative Name: BCMA

Background: B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

Molecular Weight: 8.9 kDa. Due to glycosylation, the protein migrates to 12 kDa and 15-17 kDa based on Bis-Tris PAGE result.

UniProt: Q02223