BCMA Protein (AA 1-49) (Biotin,His-Avi Tag)
Quick Overview for BCMA Protein (AA 1-49) (Biotin,His-Avi Tag) (ABIN7273945)
Target
See all BCMA (TNFRSF17) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 1-49
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Purification tag / Conjugate
- This BCMA protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Mouse BCMA/TNFRSF17 Protein
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Sequence
- Met1-Thr49
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Characteristics
- Recombinant Biotinylated Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Thr49.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse BCMA, His-Avi Tag with an affinity constant of 5.64 uM as determined in SPR assay (Biacore T200).,Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).,Biotinylated Mouse BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))
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Alternative Name
- BCMA
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Background
- B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
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Molecular Weight
- 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Bis-Tris PAGE result.
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UniProt
- O88472
Target
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