VTCN1 Protein (AA 29-258) (His-Avi Tag)
Quick Overview for VTCN1 Protein (AA 29-258) (His-Avi Tag) (ABIN7273952)
Target
See all VTCN1 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 29-258
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Purification tag / Conjugate
- This VTCN1 protein is labelled with His-Avi Tag.
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Purpose
- Human B7-H4 Protein
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Sequence
- Phe29-Ala258
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Characteristics
- Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human B7-H4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
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V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (Biotin,His-Avi Tag)
VTCN1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (His tag)VTCN1 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 33-191) (Active) protein (His tag)VTCN1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 33-194) (Active) protein (hFc Tag)VTCN1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 33-194) protein (Fc Tag)VTCN1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (Active) protein (Biotin)VTCN1 Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (Active) protein (His tag)VTCN1 Origin: Rhesus Monkey Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-256) protein (His tag)VTCN1 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 31-258) protein (His-IF2DI Tag)VTCN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 25-259) protein (His tag)VTCN1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
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Alternative Name
- B7-H4
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Background
- B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .
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Molecular Weight
- 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result.
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UniProt
- Q7Z7D3-1
Target
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