B7-H3 (4Ig) (AA 27-461) (Active) protein (Biotin,His-Avi Tag)

Catalog No. ABIN7273966

This Recombinant B7-H3 (4Ig) protein is expressed in HEK-293 Cells.

Quick Overview for B7-H3 (4Ig) (AA 27-461) (Active) protein (Biotin,His-Avi Tag) (ABIN7273966)

Target: B7-H3 (4Ig)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-461

Purification tag / Conjugate: Biotin,His-Avi Tag

Purpose: Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein

Sequence: Gly27-Thr461

Characteristics: Recombinant Biotinylated Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly27-Thr461.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-B7-H3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 8.8ng/ml determined by ELISA.,Biotinylated Human B7-H3 (4Ig) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human B7-H3 (4Ig) is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: B7-H3 (4Ig)

Background: B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig-B7-H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Molecular Weight: 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: Q5ZPR3