BST2 Protein (AA 49-161) (hFc Tag)

Catalog No. ABIN7273993

This Recombinant BST2 protein is expressed in HEK-293 Cells.

Quick Overview for BST2 Protein (AA 49-161) (hFc Tag) (ABIN7273993)

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 49-161

Purification tag / Conjugate: This BST2 protein is labelled with hFc Tag.

Purpose: Human BST2 Protein

Sequence: Asn49-Ser161

Characteristics: Recombinant Human BST2 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Asn49-Ser161.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BST2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BST2 Antibody, hFc Tag with the EC50 of 40.0ng/ml determined by ELISA. determined by ELISA.,Human BST2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human BST2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Alternative Name: BST2

Background: Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.

Molecular Weight: 40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: Q10589

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response