C5 Protein (AA 19-1676) (His tag)

Catalog No. ABIN7274038

This Recombinant C5 protein is expressed in HEK-293 Cells.

Quick Overview for C5 Protein (AA 19-1676) (His tag) (ABIN7274038)

Target: C5 (Complement Component 5 (C5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-1676

Purification tag / Conjugate: This C5 protein is labelled with His tag.

Purpose: Human Complement C5 Protein

Sequence: Gln19-Cys1676

Characteristics: Recombinant Human Complement C5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Cys1676.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).,Human Complement C5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Complement C5 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: C5 (Complement Component 5 (C5))

Alternative Name: Complement C5

Background: Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates.C5 plays a major role in complement activation, C5 convertase cleaves C5 into the pro-inflammatory C5a, and C5b, the nidus for the formation of the lytic membrane attack complex.

Molecular Weight: 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Bis-Tris PAGE result.

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis