IL2 Receptor beta Protein (AA 27-239) (His tag,Biotin)

Catalog No. ABIN7274057

Product Details

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Protein Type: Recombinant

Protein Characteristics: AA 27-239

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This IL2 Receptor beta protein is labelled with His tag,Biotin.

Purpose: Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling)

Sequence: Ala27-Asp239

Characteristics: Recombinant Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus., It contains Ala27-Asp239.

Purity: >95 % as determined by Tris-Bis PAGE, >90 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.148 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: IL-2 R beta (IL2RB Products)

Background: IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.

Molecular Weight: 25.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q38J85

Pathways: JAK-STAT Signaling, Growth Factor Binding