CD200R1 Protein (AA 27-266) (His-Avi Tag)

Catalog No. ABIN7274117

Recombinant CD200R1 protein expressed in HEK-293 Cells.

Quick Overview for CD200R1 Protein (AA 27-266) (His-Avi Tag) (ABIN7274117)

Target: CD200R1 (CD200 Receptor 1 (CD200R1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-266

Purification tag / Conjugate: This CD200R1 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human CD200 R1/CRTR2 Protein

Sequence: Ala27-Leu266

Characteristics: Recombinant Human CD200 R1/CRTR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Leu266.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD200 R1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).,Human CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD200 R1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD200R1 (CD200 Receptor 1 (CD200R1))

Alternative Name: CD200 R1

Background: CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.

Molecular Weight: 29.7 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.