CD39 Protein (AA 38-478) (His tag)

Catalog No. ABIN7274171

Recombinant CD39 protein expressed in HEK-293 Cells.

Quick Overview for CD39 Protein (AA 38-478) (His tag) (ABIN7274171)

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 38-478

Purification tag / Conjugate: This CD39 protein is labelled with His tag.

Purpose: Mouse CD39/ENTPD1 Protein

Sequence: Thr38-Ile478

Characteristics: Recombinant Mouse CD39/ENTPD1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr38-Ile478.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse CD39 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to hydrolyze the 5'-phosphate groups from the substrate adenosine-5'-triphosphate (ATP). The specific activity is >18,000-pmol/min/μg.,The purity of Mouse CD39 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris-HCl, 150 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Alternative Name: CD39

Background: CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues.CD39 is involved in the processes of thromboregulation and vascular inflammation. The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases .

Molecular Weight: 50.72 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result.

UniProt: P55772