CD28 Protein (CD28) (AA 19-152) (His-Avi Tag,Biotin)
Quick Overview for CD28 Protein (CD28) (AA 19-152) (His-Avi Tag,Biotin) (ABIN7274256)
Target
See all CD28 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 19-152
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Purification tag / Conjugate
- This CD28 protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein
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Sequence
- Asn19-Pro152
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Characteristics
- Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-CD28 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA. See testing image for detail.
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CD28 (CD28) (AA 19-152) protein (His-Avi Tag)
CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD28 (CD28) (AA 19-152) protein (mFc-His Tag)CD28 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
CD28 (CD28) (AA 19-152) protein (Fc Tag)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag,AVI tag,Biotin)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (Active) protein (His tag)CD28 Origin: Human, Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD28 (CD28) (AA 19-152) protein (mFc Tag)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD28 (CD28) (AA 18-153) protein (mFc Tag)CD28 Origin: Dog Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (AA 18-153) protein (His tag)CD28 Origin: Dog Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (Active) protein (Biotin)CD28 Origin: Human, Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD28
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Alternative Name
- CD28
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Background
- CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
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Molecular Weight
- 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result.
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UniProt
- P10747
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Target
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