SARS-CoV-2 Spike S1 Protein (AA 14-683) (His tag)

Catalog No. ABIN7274390

Recombinant SARS-CoV-2 Spike S1 protein expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike S1 Protein (AA 14-683) (His tag) (ABIN7274390)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 14-683

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His tag.

Purpose: SARS-COV-2 Spike S1 (Deltacron) Protein

Sequence: Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)

Characteristics: Recombinant SARS-COV-2 Spike S1(Deltacron) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). Alanine substitutions (R683A) are introduced to stabilize the trimeric prefusion state of SARS-COV-2 Spike S1(Deltacron) Protein and abolish the furin cleavage site, respectively.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml determined by ELISA.,SARS-COV-2 Spike S1 (Deltacron) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS ( pH 7.4).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike S1

Target Type: Viral Protein

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 73.94 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.