SARS-CoV-2 Spike S1 Protein (E484K, K417N, N501Y) (His tag)
Quick Overview for SARS-CoV-2 Spike S1 Protein (E484K, K417N, N501Y) (His tag) (ABIN7274391)
Target
See all SARS-CoV-2 Spike S1 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- E484K, K417N, N501Y
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Purification tag / Conjugate
- This SARS-CoV-2 Spike S1 protein is labelled with His tag.
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Purpose
- SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein
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Sequence
- Val16-Arg685 (N501Y,K417N,E484K)
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Characteristics
- Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685(N501Y,K417N,E484K).
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA. See testing image for detail.
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SARS-CoV-2 Spike S1 protein (His tag)
Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS, Func
SARS-CoV-2 Spike S1 (B.1.1.529 - Omicron) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (RBD) protein (His tag,AVI tag,Biotin)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
SARS-CoV-2 Spike S1 protein (Fc-Avi Tag)Origin: SARS Coronavirus (SARS-CoV) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (N501Y) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (P.1 - gamma) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (BA.4 - Omicron), (BA.5 - Omicron) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (BA.2.12.1 - Omicron) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (C.37 - Lambda) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (B.1.617.2 - delta) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
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Storage
- -80 °C
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Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- SARS-CoV-2 Spike S1
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Target Type
- Viral Protein
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Background
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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Molecular Weight
- 75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.
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NCBI Accession
- YP_009724390
Target
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