SARS-CoV-2 Spike S1 Protein (AA 16-305) (His-DYKDDDDK Tag)

Catalog No. ABIN7274392

This Recombinant SARS-CoV-2 Spike S1 protein is expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike S1 Protein (AA 16-305) (His-DYKDDDDK Tag) (ABIN7274392)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 16-305

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His-DYKDDDDK Tag.

Purpose: SARS-COV-2 Spike S1 NTD Protein

Sequence: Val16-Ser305

Characteristics: Recombinant SARS-COV-2 Spike S1 NTD Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Val16-Ser305.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: SARS-COV-2 Spike S1 NTD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-COV-2 Spike S1 NTD is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike S1

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 35 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.