CPM Protein (AA 18-423) (His tag)
Quick Overview for CPM Protein (AA 18-423) (His tag) (ABIN7274411)
Target
See all CPM ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 18-423
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Purification tag / Conjugate
- This CPM protein is labelled with His tag.
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Purpose
- Human CPM Protein
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Sequence
- Leu18-Ser423
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Characteristics
- Recombinant Human CPM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Ser423.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human CPM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde. The specific activity is >40,000 pmol/min/ug.,The purity of Human CPM is greater than 95% as determined by SEC-HPLC.
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Carboxypeptidase M (CPM) protein (His tag)
CPM Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Carboxypeptidase M (CPM) (AA 1-443) protein (GST tag)CPM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carboxypeptidase M (CPM) (AA 18-422) protein (His tag)CPM Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Carboxypeptidase M (CPM) (Active) protein (His tag)CPM Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CPM (Carboxypeptidase M (CPM))
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Alternative Name
- CPM
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Background
- Carboxypeptidase M (CPM) is a glycosylphosphatidylinositol anchored enzyme that plays an important role in the kallikrein-kinin system (KKS). CPM catalytic domain hydrolyzes Arg from C-terminal peptides (i.e., bradykinin and kallidin), generating des-Arg-kinins, the agonists of B1 receptor (B1R).
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Molecular Weight
- 47.48 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
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UniProt
- P14384
Target
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