CPM Protein (AA 18-423) (His tag)

Catalog No. ABIN7274411

Recombinant CPM protein expressed in HEK-293 Cells.

Quick Overview for CPM Protein (AA 18-423) (His tag) (ABIN7274411)

Target: CPM (Carboxypeptidase M (CPM))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-423

Purification tag / Conjugate: This CPM protein is labelled with His tag.

Purpose: Human CPM Protein

Sequence: Leu18-Ser423

Characteristics: Recombinant Human CPM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Ser423.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CPM (Carboxypeptidase M (CPM))

Alternative Name: CPM

Background: Carboxypeptidase M (CPM) is a glycosylphosphatidylinositol anchored enzyme that plays an important role in the kallikrein-kinin system (KKS). CPM catalytic domain hydrolyzes Arg from C-terminal peptides (i.e., bradykinin and kallidin), generating des-Arg-kinins, the agonists of B1 receptor (B1R).

Molecular Weight: 47.48 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

UniProt: P14384