CPM Protein (His tag)

Catalog No. ABIN7194614

Product Details

Target: CPM (Carboxypeptidase M (CPM))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CPM protein is labelled with His tag.

Purpose: Recombinant Human Carboxypeptidase M/CPM Protein (His Tag)(Active)

Sequence: Met 1-His 422

Characteristics: A DNA sequence encoding the human CPM (NP_938079.1) without the propeptide (Met 1-His 422) was expressed, fused with a polyhistidine tag at the C-terminus.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to release Larginine from BenzoylAlaArg, with detection of the arginine amino group by ophthaldialdehyde.The specific activity is >40,000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CPM (Carboxypeptidase M (CPM))

Alternative Name: Carboxypeptidase M/CPM (CPM Products)

Synonyms: im:7143022 Protein, im:7143022l Protein, si:dkey-11p6.1 Protein, zgc:110307 Protein, CPM Protein, 1110060I01Rik Protein, 5730456K23Rik Protein, AA589379 Protein, E030045M14Rik Protein, carboxypeptidase M Protein, carboxypeptidase D Protein, CPM Protein, cpm Protein, LOC5574253 Protein, Cpm Protein

Background:

Background: Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.

Synonym: Carboxypeptidase M,CPM

Molecular Weight: 47.7 kDa

NCBI Accession: NP_938079