DLL3 Protein (AA 27-490) (Biotin,His tag)

Catalog No. ABIN7274483

Recombinant DLL3 protein expressed in HEK-293 Cells.

Quick Overview for DLL3 Protein (AA 27-490) (Biotin,His tag) (ABIN7274483)

Target: DLL3 (delta Like Protein 3 (DLL3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 27-490

Purification tag / Conjugate: This DLL3 protein is labelled with Biotin,His tag.

Purpose: Biotinylated Human DLL3 Protein (Primary Amine Labeling)

Sequence: Ala27-Arg490

Characteristics: Recombinant Biotinylated Human DLL3 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus, It contains Ala27-Arg490.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human DLL3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).,Biotinylated Human DLL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human DLL3, His Tag with an affinity constant of 0.27 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DLL3 (delta Like Protein 3 (DLL3))

Alternative Name: DLL3

Background: Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Molecular Weight: 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.

UniProt: Q9NYJ7

Pathways: Notch Signaling