Ephrin A3 Protein (EFNA3) (AA 23-213) (His tag)

Catalog No. ABIN7274514

This Recombinant Ephrin A3 protein is produced in HEK-293 Cells.

Quick Overview for Ephrin A3 Protein (EFNA3) (AA 23-213) (His tag) (ABIN7274514)

Target: Ephrin A3 (EFNA3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-213

Purification tag / Conjugate: This Ephrin A3 protein is labelled with His tag.

Purpose: Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein

Sequence: Gln23-Ser213

Characteristics: Recombinant Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Ser213.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human/Cynomolgus/Rhesus macaque Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human/Cynomolgus/Rhesus macaque Ephrin-A3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Ephrin A3 (EFNA3)

Alternative Name: EFNA3

Background: Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.

Molecular Weight: 22.51 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_001252612

UniProt: P52797

Pathways: RTK Signaling