EGFRviii Protein (AA 25-378) (His-Avi Tag)
Quick Overview for EGFRviii Protein (AA 25-378) (His-Avi Tag) (ABIN7274517)
Target
Protein Type
Origin
Source
Purity
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Protein Characteristics
- AA 25-378
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Purification tag / Conjugate
- This EGFRviii protein is labelled with His-Avi Tag.
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Purpose
- Human EGFRVIII Protein
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Sequence
- Leu25-Ser378
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Characteristics
- Recombinant Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu25-Ser378.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human EGFRVIII, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.
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EGFR vIII (EGFRv3) (AA 25-378) (Active) protein (Biotin,His-Avi Tag)
EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
EGFR vIII (EGFRv3) (AA 25-378) (Active) protein (His-Avi Tag,FITC)EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
EGFR vIII (EGFRv3) (AA 25-378) (Active) protein (His-Avi Tag,PE)EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant Active
EGFR vIII (EGFRv3) (AA 25-645) protein (His tag)EGFRv3 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
EGFR vIII (EGFRv3) (AA 25-378) (Active) protein (His tag,FITC)EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
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Alternative Name
- EGFRVIII
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Background
- The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
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Molecular Weight
- 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
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NCBI Accession
- NP_001333870
Target
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