EGF Protein (AA 977-1029) (hFc Tag)

Catalog No. ABIN7274530

This Recombinant EGF protein is expressed in HEK-293 Cells.

Quick Overview for EGF Protein (AA 977-1029) (hFc Tag) (ABIN7274530)

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 977-1029

Purification tag / Conjugate: This EGF protein is labelled with hFc Tag.

Purpose: Mouse EGF Protein

Sequence: Asn977-Arg1029

Characteristics: Recombinant Mouse EGF Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Asn977-Arg1029.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 3.98 nM as determined in SPR assay (Biacore T200).,Mouse EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.4 - 3 ng/mL.,The purity of Mouse EGF is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

Molecular Weight: 32.35 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

UniProt: P01132

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation