Ephrin B2 Protein (EFNB2) (AA 28-229) (hFc Tag)

Catalog No. ABIN7274531

Recombinant Ephrin B2 protein expressed in HEK-293 Cells.

Quick Overview for Ephrin B2 Protein (EFNB2) (AA 28-229) (hFc Tag) (ABIN7274531)

Target: Ephrin B2 (EFNB2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-229

Purification tag / Conjugate: This Ephrin B2 protein is labelled with hFc Tag.

Purpose: Human Ephrin-B2/EFNB2 Protein

Sequence: Ile28-Ala229

Characteristics: Recombinant Human Ephrin-B2/EFNB2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile28-Ala229.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).,Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).,Human Ephrin B2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Ephrin B2 (EFNB2)

Alternative Name: Ephrin-B2

Background: Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.

Molecular Weight: 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_004084

Pathways: RTK Signaling, Regulation of Muscle Cell Differentiation