EFNA4 Protein (AA 26-171) (hFc Tag)

Catalog No. ABIN7274545

This Recombinant EFNA4 protein is expressed in HEK-293 Cells.

Quick Overview for EFNA4 Protein (AA 26-171) (hFc Tag) (ABIN7274545)

Target: EFNA4 (Ephrin A4 (EFNA4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-171

Purification tag / Conjugate: This EFNA4 protein is labelled with hFc Tag.

Purpose: Human Ephrin-A4/EFNA4 Protein

Sequence: Leu26-Gly171

Characteristics: Recombinant Human Ephrin-A4/EFNA4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu26-Gly171.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).,Human Ephrin-A4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Ephrin-A4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EFNA4 (Ephrin A4 (EFNA4))

Alternative Name: Ephrin-A4

Background: Ephrin A4 is one of the ephrin ligand molecules belonging to the tyrosine kinases receptor family. It was originally identified in a T-lymphoma cell line and seen to be expressed in human adult tissue as well as several tumor types. The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy.

Molecular Weight: 43.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: P52798

Pathways: RTK Signaling