EPH Receptor B2 Protein (EPHB2) (AA 19-542) (His tag)

Catalog No. ABIN7274547

This Recombinant EPH Receptor B2 protein is produced in HEK-293 Cells.

Quick Overview for EPH Receptor B2 Protein (EPHB2) (AA 19-542) (His tag) (ABIN7274547)

Target: EPH Receptor B2 (EPHB2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-542

Purification tag / Conjugate: This EPH Receptor B2 protein is labelled with His tag.

Purpose: Cynomolgus EPHB2 Protein

Sequence: Val19-Pro542

Characteristics: Recombinant Cynomolgus EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Pro542.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus EPHB2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.,Cynomolgus EPHB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus EPHB2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background: EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.

Molecular Weight: 59.09 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.

UniProt: A0A7N9CQH5

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins