EPH Receptor A3 Protein (EPHA3) (AA 21-541) (His tag)

Catalog No. ABIN7274564

Recombinant EPH Receptor A3 protein expressed in HEK-293 Cells.

Quick Overview for EPH Receptor A3 Protein (EPHA3) (AA 21-541) (His tag) (ABIN7274564)

Target: EPH Receptor A3 (EPHA3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-541

Purification tag / Conjugate: This EPH Receptor A3 protein is labelled with His tag.

Purpose: Mouse EPHA3 Protein

Sequence: Glu21-His541

Characteristics: Recombinant Mouse EPHA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-His541.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Mouse EPHA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse EPHA3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EPH Receptor A3 (EPHA3)

Alternative Name: EPHA3

Background: Erythropoietin-producing hepatocellular carcinoma cell surface type-A receptor 3 (EPHA3) has been found to promote the proliferation and survival of prostate cancer (PCa) cell lines and prostate tumor development in nude mice. The interaction of AR and SP1 contributes to regulate EPHA3 expression, and the SP1 binding sites (-295~-261) in the EPHA3 core promoter region is crucial to the regulation of EPHA3 expression in response to androgen hormone stimuli.

Molecular Weight: 59.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_034270

Pathways: RTK Signaling, Regulation of Cell Size