FGF2 Protein (AA 143-288)

Catalog No. ABIN7274613

This Recombinant FGF2 protein is produced in Escherichia coli (E. coli).

Quick Overview for FGF2 Protein (AA 143-288) (ABIN7274613)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 143-288

Purpose: Human FGF basic Protein

Sequence: Pro143-Ser288

Characteristics: Recombinant Human FGF basic Protein is expressed from E.coli without tag, It contains Pro143-Ser288.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.02 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGF basic at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 11.1ng/ml determined by ELISA.,Immobilized Human FGF basic, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 0.49 ug/ml determined by ELISA.,Human FGF basic on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 0.1 - 0.6 ng/ml (QC Test).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 8 % trehalose ( pH 7.4).

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF basic

Background: FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60 % amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

Molecular Weight: 16.5 kDa same as Bis-Tris PAGE result.

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins