FGFR1 Protein (AA 22-374) (His-Avi Tag)

Catalog No. ABIN7274633

Recombinant FGFR1 protein expressed in HEK-293 Cells.

Quick Overview for FGFR1 Protein (AA 22-374) (His-Avi Tag) (ABIN7274633)

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-374

Purification tag / Conjugate: This FGFR1 protein is labelled with His-Avi Tag.

Purpose: Human FGFR1 alpha (IIIc) Protein

Sequence: Arg22-Glu374

Characteristics: Recombinant Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu374.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGFR1 alpha (IIIc) at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Alternative Name: FGFR1 alpha

Background: Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

Molecular Weight: 42 kDa. Due to glycosylation, the protein migrates to 68-83 kDa based on Tris-Bis PAGE result.

UniProt: P11362-7

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins