FLT3 Protein (AA 27-541) (hFc Tag)

Catalog No. ABIN7274657

Recombinant FLT3 protein expressed in HEK-293 Cells.

Quick Overview for FLT3 Protein (AA 27-541) (hFc Tag) (ABIN7274657)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 27-541

Purification tag / Conjugate: This FLT3 protein is labelled with hFc Tag.

Purpose: Human FLT3/Flk-2 Protein

Sequence: Asn27-Asn541

Characteristics: Recombinant Human FLT3/Flk-2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asn27-Asn541.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Mouse FLT3 Ligand, His Tag captured on CM5 Chip via anti-his antibody can bind Human FLT3, hFc Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).,Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).,Human FLT3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT3

Background: The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.

Molecular Weight: 84.9 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.

UniProt: P36888

Pathways: RTK Signaling