FCGR1 Protein (AA 16-288) (Biotin,His-Avi Tag)

Catalog No. ABIN7274673

This Recombinant FCGR1 protein is produced in HEK-293 Cells.

Quick Overview for FCGR1 Protein (AA 16-288) (Biotin,His-Avi Tag) (ABIN7274673)

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 16-288

Purification tag / Conjugate: This FCGR1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Fc gamma RI/CD64 Protein

Sequence: Gln16-Pro288

Characteristics: Recombinant Biotinylated Human Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln16-Pro288.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay (Biacore T200).,Biotinylated Human Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Biotinylated Human Fc gamma RI, His-Avi Tag on Anti-His-Biosensor can bind Trastuzumab with an affinity constant of 2.94 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human Fc gamma RI is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Alternative Name: Fc gamma RI

Background: The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

Molecular Weight: 33.5 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result.

UniProt: P12314

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process