FcRn Protein (AA 22-297) (His tag)

Catalog No. ABIN7274677

This Recombinant FcRn protein is expressed in HEK-293 Cells.

Quick Overview for FcRn Protein (AA 22-297) (His tag) (ABIN7274677)

Target: FcRn (neonatal Fc Receptor (FcRn))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-297

Purification tag / Conjugate: This FcRn protein is labelled with His tag.

Purpose: Mouse FcRn Protein

Sequence: Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)

Specificity: Uni-Prot: Q61559 (FCGRT), P01887 (B2M)

Characteristics: Recombinant Mouse FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ser22-Ser297 (FCGRT) and Ile21-Met119 (B2M).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (Biacore T200) (QC Test).,Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 20.82 nM as determined in SPR assay (Biacore T200).,Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).,Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).,Mouse FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse FcRn is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FcRn (neonatal Fc Receptor (FcRn))

Alternative Name: FcRn

Background: The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Molecular Weight: 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result.

UniProt: Q61559

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process