GUCY2C Protein (AA 24-430) (His-Avi Tag)

Catalog No. ABIN7274716

This Recombinant GUCY2C protein is expressed in HEK-293 Cells.

Quick Overview for GUCY2C Protein (AA 24-430) (His-Avi Tag) (ABIN7274716)

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-430

Purification tag / Conjugate: This GUCY2C protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human GUCY2C/Guanylyl cyclase C Protein

Sequence: Ser24-Gln430

Characteristics: Recombinant human GUCY2C/Guanylyl cyclase C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser24-Gln430.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GUCY2C, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GUCY2C Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.,Human GUCY2C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GUCY2C is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Alternative Name: GUCY2C

Background: Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial dynamics, antitumorigenesis, and intestinal barrier function. GUCY2C may represent a new target for anti-obesity pharmacotherapy.

Molecular Weight: 48.8 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: P25092