GDF15 Protein (AA 193-308) (His tag)

Catalog No. ABIN7274718

This Recombinant GDF15 protein is produced in Escherichia coli (E. coli).

Quick Overview for GDF15 Protein (AA 193-308) (His tag) (ABIN7274718)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 193-308

Purification tag / Conjugate: This GDF15 protein is labelled with His tag.

Purpose: Cynomolgus GDF15 Protein

Sequence: Arg193-Val308

Characteristics: Recombinant Cynomolgus GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Arg193-Val308.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus GDF15, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 39.9 ng/ml determined by ELISA (QC Test).,Immobilized Cynomolgus GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 6.1 ng/ml determined by ELISA.,Cynomolgus GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM HAc ( pH 2.9). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM HAc ( pH 2.9). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

Molecular Weight: 14.15 kDa. The protein migrates to 15-18 kDa based on Bis-Tris PAGE result.

UniProt: G7PWZ3

Pathways: SARS-CoV-2 Protein Interactome