GDF15 Protein (AA 193-308) (His tag)

Catalog No. ABIN7274718

Product Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Protein Characteristics: AA 193-308

Origin: Cynomolgus

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GDF15 protein is labelled with His tag.

Purpose: Cynomolgus GDF15 Protein

Sequence: Arg193-Val308

Characteristics: Recombinant Cynomolgus GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Arg193-Val308.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus GDF15, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 56.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM HAc ( pH 2.9).

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM HAc ( pH 2.9). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15 (GDF15 Products)

Synonyms: GDF15 Protein, GDF-15 Protein, MIC-1 Protein, MIC1 Protein, NAG-1 Protein, PDF Protein, PLAB Protein, PTGFB Protein, SBF Protein, growth differentiation factor 15 Protein, GDF15 Protein, Gdf15 Protein

Background: Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

Molecular Weight: 14.15 kDa. The protein migrates to 15-18 kDa based on Tris-Bis PAGE result.

UniProt: G7PWZ3

Pathways: SARS-CoV-2 Protein Interactome